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Year : 2021  |  Volume : 1  |  Issue : 1  |  Page : 154-155

Usefulness of mitochondrial deoxyribonucleic acid barcoding in identification of Chrysomya bezziana ophthalmomyasis: A case report

1 Department of Orbit and Oculoplasty, Aravind Eye Hospital and PG Institute of Ophthalmology, Madurai, Tamil Nadu, India
2 Department of Paediatric Ophthalmology, Aravind Eye Hospital and PG Institute of Ophthalmology, Madurai, Tamil Nadu, India
3 Scientist ‘F’ and Officer in Charge ICMR-Vector Control Research Centre, Indian Council of Medical Research, Department of Health Research, Ministry of Health and Family Welfare, Madurai, Tamil Nadu, India

Correspondence Address:
Dr. Meghana Tanwar
Aravind Eye Hospital and PG Institute of Ophthalmology, 1, Anna Nagar Main Road, Sathamangalam, Madurai - 625 020, Tamil Nadu
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ijo.IJO_1758_20

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We report a case of ophthalmomyiasis profunda with a complete destruction of ocular tissues in an old diabetic lady who presented with sudden onset of bleeding from a progressively increasing, painless swelling in her left eye since two weeks. She was primarily treated with Ivermectin, paraffin dressing, manual removal of maggots, and then total exenteration. Deoxyribonucleic acid (DNA) barcoding analysis supported by DNA sequencing accurately identified the species as Chrysomya bezziana based on the mitochondrial cytochrome c oxidase 1 gene. This case report demonstrates the usefulness of DNA barcoding, complementing the conventional identification methods, in accurate identification of maggots of ophthalmic significance.

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